repository of small ORFs identified by ribosome profiling

This section provides a summary on the acquisition and processing of the data. For more detailed information, feel free to consult the available literature. Use the navigation bar on the left to navigate trough the different steps.

7. ORFscore

The ORFscore calculates the preference of RPFs to accumulate in the first frame of coding sequences.7 To compute the ORFscore RPFs are counted in each frame (in-frame,+1 frame,+2 frame). Next the distribution of RPFs is compared to an equally sized uniform distribution using a modified chi-squared statistic. Only RPFs with fragment lengths corresponding to the most abundant, in-frame RPFs found in canonical protein coding transcripts, are used.